Detecting the region of interest plays an important role in the field of image processing and analysis. For the
microscopic image of plant embryo slice, region of interest usually indicates various cells or macromolecules.
Combining contour evolution theory and pulse coupled neural network, we propose a new method of macromolecular
detection and extraction for biological microscopic image. Some existing methods are compared with the proposed
method. Experimental results show the proposed method has the better performance than existing methods.