To study the preventive and therapeutic effects of Allium Macrostemon Powder (AP) on experimental ulcerative colitis and its antioxidant and anti-inflammatory effects in vitro. Sodium dextran sulfate (DSS) was used to establish the model of ulcerative colitis in mice. Lipopolysaccharide (LPS) was used to induce cell inflammation. The scavenging capacity of AP on ABTS and DPPH free radicals was measured in vitro. The results showed that the drug had no significant effect on colitis, but cell experiments in vitro showed that it had obvious anti-inflammatory effect, and had good scavenging ability on ABTS and DPPH free radicals. AP has anti-inflammatory and antioxidant effects.
Vespae nidus has been used in traditional Chinese medicine for thousands of years to treat a variety of diseases, including malignant tumors, rheumatoid arthritis, lung diseases, skin disease, digestive and urinary disorders, and dental diseases. As the same, Epimedii folium used to treat kidney-Yang deficiency,impotence and seminal emission, limp wilting sinews and bones, rheumatic arthralgia, Numbness and paralysis, osteoporosis. Vespae nidus-Epimedii folium formula used to treat chronic arthralgia due to Yang deficiency, and painful joints. But the underlying mechanisms by which it exerts therapeutic function have not been thoroughly studied. Cellular damage induced by free-radicals like reactive oxygen species has been implicated in several diseases. Hence naturally anti-oxidant play a vital role in combating these conditions. In this study, The effects of Vespae Nidus nidus-Epimedii folium formula aqueous extract (VEF) on free radical scavenging and anti-oxidative capacity is investigated. By free radical scavenging assays, the IC50 value of VEF were 71.33, 54.07 μg/mL with DPPH, ABTS assay respectively, and 0.161mg ferrous sulfate/1 mg VEF with FRAP assay. These results indicated that the antioxidant properties of VEF hold great potential used as an alternative to more toxic synthetic anti-oxidants as an additive in food, cosmetic and pharmaceutical preparations for the oxidative diseases treatment.
KEYWORDS: Decision support systems, In vitro testing, Animal model studies, Life sciences, Inflammation, Colon, Absorbance, Statistical analysis, Sodium, Water
To explore the effect of Coptis chinensis-Scutellaria baicalensis on ulcerative colitis in mice and its antioxidant and anti-inflammatory effects in vitro. Sodium dextran sulfate (DSS) was used to induce acute ulcerative colitis in mice, lipopolysaccharide (LPS) was used to induce cell inflammation model, and ABTS and DPPH models were used to evaluate antioxidant capacity. The results showed that the drug had no significant effect on colitis, but cell experiments in vitro showed that the drug had significant anti-inflammatory effect and could scavenge ABTS and DPPH free radicals. Coptis chinensis-scutellaria baicalensis has anti-inflammatory and anti-oxidative effects.
Guigan longmu Decoction(GL), take in Zhong-jing Zhang’s Treatise on Exogenous of Febrile Diseases, composed of Cinnamomum cassia Presl(Guizhi), Glycyrrhiza uralensis Fisch.(Gancao), Fossil fragments(Longgu) and Oyster shell(Muli), is a traditional Chinese medicine(TCM) formula and is widely used as a clinically medication formula for its efficiency in improving sleep. But the underlying mechanisms by which it exerts therapeutic function have not been thoroughly studied. Cellular damage induced by free-radicals like reactive oxygen species has been implicated in several diseases. Hence naturally anti-oxidant play a vital role in combating these conditions. In this study, The effects of GL on free radical scavenging and anti-oxidative capacity is investigated. By free radical scavenging assays, the IC50 values of GL were 46.03±1.26, 51.45±0.74 μg/ml with DPPH, ABTS assay respectively, and 0.611 mg ferrous sulfate/1 mg GL with FRAP assay. These results indicated that the antioxidant properties of GL hold great potential used as an alternative to more toxic synthetic anti-oxidants as an additive in food, cosmetic and pharmaceutical preparations for the oxidative diseases treatment.
Huzhentongfeng (HZTF) is a compound extract developed by Guangzhou (Jinan) Biomedical Research and Development Center for treating gout. To investigate the antioxidative activity and anti-inflammatory activity of HZTF, the scavenging activity of hydroxyl radical, ferrous ions chelating ability and ferric reducing antioxidant power (FRAP) were used as indicators to evaluate the antioxidant activity and the lipopolysaccharide(LPS)-induced RAW264.7 macrophages were used to assess its anti-inflammatory activity. The results showed that HZTF had certain antioxidant activity, the IC50 values of HZTF in hydroxyl radical scavenging activity and ferrous ions chelating ability are 163.83 ± 10.49 and 899.72 ± 20.98 μg/mL, respectively and the FRAP value of HZTF are 8.97 ± 0.63 mol FeSO4/g. Moreover, HZTF significantly reduced IL-1β, IL-6, TNF-α, iNOS expression and NO production in LPS-induced RAW264.7 macrophages (P < 0.05), which demonstrate that HZTF have good potential to be developed as antioxidant or anti-inflammatory medicines.
Ulcerative colitis (UC) is a chronic and refractory inflammatory disorder of the colon and rectum with increasing morbidity in recent years. Lian-ai jian (LAJ), a Chinese medicinal herb decoction, including two herbs: Coptidis Rhizoma and Artemisiae Argyi Folium. We group them according to the ancient Chinese medical books "Songfengshuoyi", and investigate the anti-inflammatory, antioxidant activity and protective effect on UC of this compound. Dextran sulfate sodium salt (DSS)-induced colitis mice were used to assess its protective effect on UC. We also determined the anti-inflammatory ability of LAJ by measuring the inhibition of IL-1β, IL-6 and TNF-α expression in LPS stimulated RAW 264.7 macrophages. The inhibition of Nitric oxide (NO) production, iNOS expression in RAW 264.7 macrophages and extracellular NO scavenging ability were used as indicators to evaluate the antioxidant activity of LAJ. The results showed that LAJ had strong anti-inflammatory, antioxidant activity and reduced the disease activity index and colon shortening of mice.
To give some foundation for quality standards of the aloe extracts, by studying the contents of the aloin in aloe extracts and evaluating scientifically the quality of the aloe extracts from different companies. The chromatographic separation was performed on a SunFire C18 column (250mm×4.6mm, 5 μm). The mobile phase comprised acetonitrile-water (25:75, V/V,) at a flow rate of 1.0 mL/min. The detection wavelength was set at 355nm. There was a great difference in the aloe extracts from different companies. There was a good linear relationship between peak area and the injection volume over the range of 2.5-20μL (R2 = 0.9992). The average spike recovery of the aloin was 99.62%, With a RSD of 1.20%(n=6). The contents of aloe extracts from different companies were 90.64-263.11mg/g. This method is simple, fast and the result is accurate and reliable and can be used for the determination of the aloin in aloe extracts.
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