Ms. Zipei Wu Profile

Zipei Wu

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 18 March 2024 Paper

Monitoring the life cycle stages of Haematococcus pluvialis by using FLIM

Yifeng Deng, Changjiang Li, Zeyu Xiao, Jiakai Chen, Zipei Wu, Xiao Peng, Zhen Lu, Wei Yan, Junle Qu

Proceedings Volume 13104, 1310470 (2024) https://doi.org/10.1117/12.3023913

KEYWORDS: Fluorescence, Fluorescence lifetime imaging, Picosecond phenomena, Confocal microscopy, Pigments, Biological samples, Time correlated single photon counting, Nondestructive evaluation, Fluorescence intensity, Autofluorescence

Read Abstract +

Haematococcus pluvialis is one type of freshwater unicellular microalgae, belonging to the Chlorophyta, Haematococcus. H. pluvialis has high economic value due to its wide variety of nutritional components, including astaxanthin, vitamins, algal polysaccharides, proteins, etc. Current microalgae detection methods are deficient in monitoring the life cycle of H. pluvialis. In this study, we applied the confocal fluorescence microscopy and the fluorescence lifetime imaging microscopy (FLIM) system to observe the different stages of the microalgal cells. The cell morphology and the fluorescence lifetime distributions were obtained for further analysis. The results showed that H. pluvialis had a diameter of about 25μm in the state of flagellated macrozooids (zoospore). When the nutrients in the medium were continuously consumed, the individual cells of H. pluvialis became larger and entered the microzooid stage, whose diameters were around 35μm. The fluorescence lifetime values of H. pluvialis in the macrozooid (zoospore) stage and the microzooid stage were around 700 to 800ps and 1000ps, respectively. The next stage was the palmella stage, in which the diameter of H. pluvialis increased to the range of 40 to 52μm. During this stage, the larger algae began to accumulate red pigment in the center of the cell. Meanwhile, the fluorescence lifetime values near the center of the algal cell increased to around 1300ps, approximately 200ps higher than the values in the periphery of the cells, showing layered properties of H. pluvialis in the palmella stage. In summary, FLIM can provide a real-time quantitative method for monitoring different life stages and cell morphologies of H. pluvialis during its life cycle in a non-destructive way.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years