Objective: To investigate the distribution of ALA in Cervical lesion tissue by tissue frozen section and fluorescence microscopy. Methods: 20% of 5-ALA were topically applied to the cervix.Biopsies were taken from the lesions for histology examination, Fluorescence microscopy was performed methods in these frozen section of cervical tissue to observe the distribution of ALA and the result was analyzed semi-quantitatively. Results: The maximal Protoporphyrin IX (PpIX) fluorescence was observed in the most serious tissue with cervical lesions .fluorescence photometry corroborated these results. Conclusion: ALA is selectively accumulated in cervical lesions, and the more severe the lesion, the easier it is to accumulate.
A total of 62 female patients who underwent ALA-PDT at the Gynecology Department of Henan Province People's Hospital in 2018 were retrospectively assessed. Lesions included 24 case of LSIL, 13 case of HSIL, 12 case of VAIN, 2 case of VIN and 11 case of VL. Three-six sessions of ALA-PDT were carried out at intervals of ten days. The treatment efficacy was evaluated by colposcopy, TCT and HPV dectection. After one month follow-up, The effective rate of LSI, HSIL, VAIN, VIN and VL were 70.8%, 84.6%, 83.3%, 100% and 100%. After three month follow-up, The effective rate of LSIL, HSIL, VAIN, VIN and VL were 83.3%, 92.3%, 83.3%, 100% and 100%. After six month follow-up, The effective rate of LSIL, HSIL, VAIN, VIN and VL were 87.5%, 92.3%, 91.7%, 100% and 100%. Our experience show that ALA-PDT is an effective treatment modality for female genital precancerous lesions. Keywords: 5-aminolevulinic acid photodynamic therapy, Low-grade cervical squamous intraepithelial lesions,