Visible resonant Raman (VRR) spectroscopy provides an effective way to enhance Raman signal from particular
bonds associated with key molecules due to changes on molecular level. This paper reports on the VRR use for
detection of human brain the control and gliomas of three grades. From the RR spectra additional two molecular
vibrational biomarkers at 1129cm-1 and 1338cm-1, for the four types of brain tissues are significantly different in
intensity. The new RR spectral peaks can be used as molecular biomarkers to evaluate glioma grades and identify
the margin of gliomas from the controls. The metabolic process of glioma cells based on the RR spectral changes
may reveal the Warburg hypothesis.