Non-uniformity noise is one of the critical problems that need to be solved in imaging system. Due to the non-uniform of each detector, there are regular strip noises in the image, which has serious effect on the image quality and thus on image applications. In target recognition system, there is also intensity difference between the adjacent images in the sequencial images other than the uniformity between each image. In this paper, a new non-uniformity correction method for sequence-image based on local histogram specification is proposed. The process is aimed to suppress the non-uniformity noise and smooth the image. The two adjacent images are interlaced into one image column by column. And then, the image is processed by the local weight histogram specification method . Experimental results show that the method presented in this paper can effectively suppress the strip noise in each image and thus reduce the gray level difference among the image sequences.