Biofuel cells are a subset of fuel cells that employ biocatalysts. Enzyme-based biofuel cells (EBFCs) generate
electrical energy from biofuels such as glucose and ethanol, which are renewable and sustainable energy sources.
Glucose biofuel cells (GBFCs) are particularly interesting nowadays due to continuous harvesting of oxygen and
glucose from bioavailable substrates, activity inside the human body, and environmental benign, which generate
electricity through oxidation of glucose on the anode and reduction of oxygen on the cathode. Promoting the
electron transfer of redox enzymes at modified electrode utilizing Nano size materials, such as carbon nanotubes
(CNT), to achieve the direct electrochemistry of enzymes has been reported. The polypyrrole-MWCNTs-glucose
oxidase (PY-CNT-GOx) electrode has been investigated in the present work. Cyclic voltammetry tests were
performed in a three-electrode electrochemical set-up with modified electrode (Pt/PPy/MWCNTs/GOx) was used
as working electrode. Platinum flat and Ag/AgCl (saturated KCl) were used as counter electrode and the reference
electrode, respectively. The biofuel cells probe was prepared by immobilizing MWCNTs at the tip of a platinum
(Pt) electrode (0.5 cm2) with PPy as the support matrix We have demonstrated a well-dispersed nanomaterial
PPy/MWNT, which is able to immobilize GOx firmly under the condition of the absence of any other cross-linking
Tamarisk usage as a new group of lignocelluloses material to produce fermentable sugars in bio ethanol process was studied. The overall aim of this work was to establish the optimum condition for acid hydrolysis of this new material and a mathematical model predicting glucose release as a function of operation variable. Sulfuric acid concentration in the range of 20 to 60%(w/w), process temperature between 60 to 95oC, hydrolysis time from 120 to 240 min and solid content 5,10,15%(w/w) were used as hydrolysis conditions. HPLC was used to analysis of the product. This analysis indicated that glucose was the main fermentable sugar and was increase with time, temperature and solid content and acid concentration was a parabola influence in glucose production. The process was modeled by a quadratic equation. Curve study and model were found that 42% acid concentration, 15 % solid content and 90oC were optimum condition.