The normal Raman spectrum and surface-enhanced Raman scattering (SERS) spectrum of herba houttuyniae decoction (HHD) were tested and analyzed. The characteristic SERS bands of HHD were tentatively assigned. There was no Raman signal in normal Raman spectrum of HHD. However, as a result of the silver colloid enhanced effects on the Raman scattering of HHD, we observed that the SERS spectrum of HHD had primary thirteen SERS peaks such as 538, 620, 686, 730, 955, 1030, 1231, 1240, 1325, 1400, 1472, 1564 and 1651 cm-1, and there were six strong signals at 538, 620, 730, 955, 1325 and 1400 cm-1 bands. The results showed that the SERS spectroscopy might provide a new kind of high-sensitive, accurate, easy, and rapid detecting method for traditional Chinese medicine (TCM).