A dual-wavelength pulse oximetry system combined with laser Doppler was developed for the assessment of perfusion. Red and infrared PPG and Doppler signals were recorded from a healthy volunteer in three studies at different measurement sites to investigate the interference between PPG and laser Doppler flowmetry (LDF). Good quality photoplethysmographic (PPG) and Doppler signals were detected simultaneously using this combined probe from the skin of the finger. The influence of the PPG light sources on LDF measurements was investigated; also the influence of the LDF light sources to the PPG measurements was studied. In the worst case, the apparent change in PPG amplitude when the LDF system was switched on was less than 8%, and the change in LDF flux amplitude when the PPG system was switched on was 14.7%.