We report the growth of gold nanostructured thin films by cylindrical direct current magnetron sputtering at room temperature. The grown gold nanostructures are mostly rod shape with short lengths of <20 nm and show negative optical parameters in the UV–vis region. So far negative permittivity and permeability were only reported for complex artificial structures. In a case of simple structures such as gold nanostructures, the negative optical parameters were only predicted by simulation methods and considered ideal structures. The small size of gold nanoparticles and the thickness of nanostructured thin films could be the reason of negative permittivity and permeability in our case. The optical properties of the samples were defined experimentally by ultraviolet-visible spectroscopy and their quality was assessed through multitechnique characterization using transmission electron microscopy, field emission scanning electron microscopy, x-ray diffraction, and energy dispersed x-ray.