Dr. Qiumei Zhou Profile

Dr. Qiumei Zhou

at China Pharmaceutical Univ

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Publications (2)

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Proceedings Article | 22 April 2016 Paper

Detection of MDR1 mRNA expression with optimized gold nanoparticle beacon

Qiumei Zhou, Zhiyu Qian, Yueqing Gu

Proceedings Volume 9723, 972311 (2016) https://doi.org/10.1117/12.2220035

KEYWORDS: Gold, Nanoparticles, Ultrasonography, Luminescence, Resistance, Confocal microscopy, Microscopes, Tumors, Cancer, Absorption

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MDR1 (multidrug resistance geneⅠ) mRNA expression is a promising biomarker for the prediction of doxorubicin resistance in clinic. However, the traditional technical process in clinic is complicated and cannot perform the real-time detection mRNA in living single cells. In this study, the expression of MDR1 mRNA was analyzed based on optimized gold nanoparticle beacon in tumor cells. Firstly, gold nanoparticle (AuNP) was modified by thiol-PEG, and the MDR1 beacon sequence was screened and optimized using a BLAST bioinformatics strategy. Then, optimized MDR1 molecular beacons were characterized by transmission electron microscope, UV–vis and fluorescence spectroscopies. The cytotoxicity of MDR1 molecular beacon on L-02, K562 and K562/Adr cells were investigated by MTT assay, suggesting that MDR1 molecular beacon was low inherent cytotoxicity. Dark field microscope was used to investigate the cellular uptake of hDAuNP beacon assisted with ultrasound. Finally, laser scanning confocal microscope images showed that there was a significant difference in MDR1 mRNA expression in K562 and K562/Adr cells, which was consistent with the results of q-PCR measurement. In summary, optimized MDR1 molecular beacon designed in this study is a reliable strategy for detection MDR1 mRNA expression in living tumor cells, and will be a promising strategy for in guiding patient treatment and management in individualized medication.

Proceedings Article | 17 September 2014 Paper

Visual detection of multidrug resistance gene in living cell using the molecular beacon imaging

Qiumei Zhou, Yi Ma, Yueqing Gu

Proceedings Volume 9230, 92301U (2014) https://doi.org/10.1117/12.2068780

KEYWORDS: Luminescence, Resistance, Cancer, Tumors, Gold, Nanoparticles, Absorption, Visualization, Oncology, Fluorescence spectroscopy

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A major problem in cancer treatment is the development of resistance to chemotherapeutic agents in tumor cells. Detection of effective prognostic biomarkers and targets are of crucial importance to the management of individualized therapies. However, quantitative analysis of the drug resistance gene had been difficult because of technical limitations. In this study, we designed and used a special hairpin deoxyribonucleic acid (DNA), which served as a beacon for detecting human drug resistance indicater. Upon hybridizing with the target mRNA, the hairpin DNA modified gold nanoparticle beacons (hDAuNP beacons) release the fluorophores attached at 5’end of the oligonucleotide sequence. The fluorescence properties of the beacon before and after the hybridization with the complementary DNA were confirmed in vitro. The hDAuNP beacons could be taken up by living cells with low inherent cytotoxicity and higher stability. hDAuNP beacon imaged by confocal laser scanning microscopy to detect the resistance gene expression. The detected fluorescence in MCF7and MCF7/ADR cells correlates with the specific drug resistance gene expression, which is consistent with the result from Q-PCR. Thus, this approach overcame many of the challenges of previous techniques by creating highly sensitive and effective intracellular probes for monitoring gene expression.

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