Calibration of a fluorescence hyperspectral imaging system for agricultural inspection and detection

Ambrose E. Ononye; Haibo Yao; Zuzana Hruska; Russell Kincaid

doi:10.1117/12.850370

20 April 2010 Calibration of a fluorescence hyperspectral imaging system for agricultural inspection and detection

Ambrose E. Ononye, Haibo Yao, Zuzana Hruska, Russell Kincaid

Proceedings Volume 7676, Sensing for Agriculture and Food Quality and Safety II; 76760E (2010) https://doi.org/10.1117/12.850370
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

Fluorescence hyperspectral imaging is increasingly being used for food quality inspection and detection of potential food safety concerns. The flexible nature of a self-scanning pushbroom hyperspectral imager lends itself to these kinds of applications, among others. To increase the use of this technique there has been a tendency to use low cost off-the-shelf hyperspectral sensors which are typically not radiometrically calibrated. To ensure that these systems are optimized for response and repeatability, it is imperative that the systems be both radiometrically and spectrally calibrated specifically for fluorescence imaging. Fluorescence imaging provides several challenges such as low signal, stray light and a low signal dynamic range that are improved with careful radiometric calibration. A radiometric and spectral approach that includes flat fielding and the conversion of digital number responses to radiance for calibrating this imaging system and other types of hyperspectral imagers is described in this paper. Results show that this method can be adopted for calibrating fluorescence and reflective hyperspectral imaging systems in the visible and near infra-red domains.

Citation Download Citation

Ambrose E. Ononye, Haibo Yao, Zuzana Hruska, and Russell Kincaid "Calibration of a fluorescence hyperspectral imaging system for agricultural inspection and detection", Proc. SPIE 7676, Sensing for Agriculture and Food Quality and Safety II, 76760E (20 April 2010); https://doi.org/10.1117/12.850370

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available