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30 March 2023 Insights developed into nuclear mechanics by live cell imaging
Tanmay P. Lele
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Proceedings Volume PC12381, Optical Elastography and Tissue Biomechanics X; PC123810C (2023) https://doi.org/10.1117/12.2655717
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
The nucleus is the largest organelle in the cell. When deformed with techniques like Atomic Force Microscopy or micropipette aspiration, the nucleus appears to be elastic and much stiffer than the cytoplasm. Whether the nucleus behaves like a stiff elastic object when shaped by cellular forces and on physiological time scales, such as during migration through confining channels, is not clear. Here I will discuss our efforts to understand nuclear mechanics in cell migration. I will present live cell imaging experiments that reveal surprising nuclear mechanical behaviors such as drop-like deformation. I will show how the nucleus is likely shaped by viscous coupling between the nucleus and the cytoplasm rather than static cytoskeletal stresses. I will conclude with an example of high content imaging of cancer nuclear morphology for drug discovery applications.
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Tanmay P. Lele "Insights developed into nuclear mechanics by live cell imaging", Proc. SPIE PC12381, Optical Elastography and Tissue Biomechanics X, PC123810C (30 March 2023); https://doi.org/10.1117/12.2655717
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KEYWORDS
Live cell imaging
Mechanics
Atomic force microscopy
Cancer
Drug discovery
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