Stimulated Raman photothermal (SRPT) microscopy (Conference Presentation)

Yifan Zhu; Xiaowei Ge; Le Wang; Ji-Xin Cheng

doi:10.1117/12.2648494

15 March 2023 Stimulated Raman photothermal (SRPT) microscopy (Conference Presentation)

Yifan Zhu, Xiaowei Ge, Le Wang, Ji-Xin Cheng

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920K (2023) https://doi.org/10.1117/12.2648494
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The detection sensitivity of SRS microscopy is fundamentally limited by shot noise. To address this barrier, we revisit SRS from the perspective of energy deposition. The SRS process pumps molecules to their vibrational excited states. The thereafter relaxation heats up the surrounding and induces a change in refractive index, as suggested by simulation and fluorescence thermometer measurement. By introducing a CW laser beam to probe the refractive index change, we demonstrate stimulated Raman photothermal (SRPT) imaging of particles, cells, and tissues with high signal to noise ratio at the fingerprint region. SRPT microscopy opens a new way to vibrational spectroscopic imaging with ultrahigh sensitivity.

Conference Presentation

Citation Download Citation

Yifan Zhu, Xiaowei Ge, Le Wang, and Ji-Xin Cheng "Stimulated Raman photothermal (SRPT) microscopy (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920K (15 March 2023); https://doi.org/10.1117/12.2648494

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