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13 March 2024 Imaging of tumor spheroids embedded in a collagen matrix with a combined optical coherence microscopy and confocal microscopy system
Santosh Balakrishnan, Meg McFetridge, Brittany Schutrum, Yuechuan Lin, Claudia Fischbach, Steven G. Adie
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Proceedings Volume PC12834, Multimodal Biomedical Imaging XIX; PC1283403 (2024) https://doi.org/10.1117/12.3001739
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
A combined 1300 nm Optical Coherence Microscopy (OCM) and 488 nm confocal reflectance/ fluorescence microscopy system was designed to perform high-resolution, high-specificity imaging of collagen-embedded spheroids. Spheroids of Hyaluronic Acid (HA) synthase-overexpressing breast epithelial cells alone, or co-cultured with adipose stromal cells were imaged. The volumes, acquired either after fixing and staining or longitudinally with labeling, enabled the visualization of the spheroid morphology, luminal structures, cellular organization, and collagen matrix remodeling. The morphology and internal lumen structures of spheroids, as large as 500 μm in diameter, could be obtained from the OCM volumes, even in the presence of dense collagen matrix surrounding the spheroids. The confocal stacks provided superior specificity to discriminate cells from the compacted collagen along the spheroid’s periphery, up to a depth of ~120 μm. The combined use of OCM and confocal imaging on these spheroid models has added to our understanding of how HA may contribute to tumor initiation and invasion.
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Santosh Balakrishnan, Meg McFetridge, Brittany Schutrum, Yuechuan Lin, Claudia Fischbach, and Steven G. Adie "Imaging of tumor spheroids embedded in a collagen matrix with a combined optical coherence microscopy and confocal microscopy system", Proc. SPIE PC12834, Multimodal Biomedical Imaging XIX, PC1283403 (13 March 2024); https://doi.org/10.1117/12.3001739
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KEYWORDS
Tumors
Confocal microscopy
Imaging systems
Collagen
Matrices
Optical coherence microscopy
3D modeling
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