Multiphoton bio-imaging: how modern femtosecond lasers enable new advancements

Ian A. Read

doi:10.1117/12.3003310

13 March 2024 Multiphoton bio-imaging: how modern femtosecond lasers enable new advancements

Ian A. Read

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC1284705 (2024) https://doi.org/10.1117/12.3003310
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Multiphoton microscopy has seen widespread adoption from the research community to address the challenges associated with image collection many hundreds of micrometers into living tissue. Over time, technology advancements have contributed to the evolution of 2-photon, 3-photon and other nonlinear microscopy techniques. Deep Tissue imaging necessitates wavelengths spanning from the visible to the near-IR to achieve desirable resolution. Excitation requires a femtosecond laser pulse to minimize out-of-focus fluorescence. Image collection requires novel broadband microscope objectives which must transmit both the near-IR excitation wavelengths and the subsequent visible fluorescence. During this presentation, a concise overview of the historical development of these technologies will be presented through real-life examples that demonstrate how modern research facilities are leveraging these advancements.

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Citation Download Citation

Ian A. Read "Multiphoton bio-imaging: how modern femtosecond lasers enable new advancements", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC1284705 (13 March 2024); https://doi.org/10.1117/12.3003310

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KEYWORDS

Multiphoton microscopy

Deep tissue imaging

Fluorescence

Femtosecond pulse shaping

Image resolution

Image transmission

Microscopes

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