Feasibility of using spatial frequency domain imaging to aid in fluorescence guided resection (Conference Presentation)

Dennis J. Wirth; Mira Sibai; David W. Roberts M.D.; Keith D. Paulsen

doi:10.1117/12.2291134

5 April 2018 Feasibility of using spatial frequency domain imaging to aid in fluorescence guided resection (Conference Presentation)

Dennis J. Wirth, Mira Sibai, David W. Roberts M.D., Keith D. Paulsen

Author Affiliations +

Proceedings Volume 10484, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI; 104840J (2018) https://doi.org/10.1117/12.2291134
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The goal of fluorescence-guided surgery (FGR) is to provide real-time enhancement of tumors to maximize safe resection. The optical property mapping ability of spatial frequency domain imaging (SFDI) has enabled quantitative fluorescence imaging (qFI) of protoporphyrin IX (PpIX) in gliomas in the pre-clinical setting. The goal of this study was to evaluate the feasibility of using SFDI to allow for qFI to enhance FGR. Specifically, we modified a benchtop SFDI system to mount directly to a commercial surgical microscope(Zeiss). A commercially available digital light processing module (DLI Austin, TX) was used to modulate light from a xenon arc lamp to illuminate the field. White light excitation and a liquid crystal tunable filter (LCTF Verispec) was used to measure diffuse reflectance at discreet wavelengths from 420 nm to 720 nm on a CMOS camera. An illumination side filter wheel allowed for excitation of PpIX fluorescence at 405 nm and 635 nm and the LCTF measured fluorescence emission at 670 nm and 710 nm. The ability of the clinical microscope to perform optical mapping and qFI was tested with tissue simulating phantoms and live mouse models. The results of these tests showed that SFDI can be implemented in a clinical microscope and the optical mapping and qFI abilities of SFDI may be used to enhance FGR.

Conference Presentation

Citation Download Citation

Dennis J. Wirth, Mira Sibai, David W. Roberts M.D., and Keith D. Paulsen "Feasibility of using spatial frequency domain imaging to aid in fluorescence guided resection (Conference Presentation)", Proc. SPIE 10484, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI, 104840J (5 April 2018); https://doi.org/10.1117/12.2291134

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Luminescence

Tissue optics

Microscopes

Digital Light Processing

Modulation

Optical properties

Reflectivity

Show All Keywords

Keywords/Phrases

Search In:

Publication Years