(Re)defining sensitivity of chemical imaging

Sean P. O'Connor; Joshua Lalonde; Dawson T. Nodurft; Vladislav V. Yakovlev

doi:10.1117/12.2510974

4 March 2019 (Re)defining sensitivity of chemical imaging

Sean P. O'Connor, Joshua Lalonde, Dawson T. Nodurft, Vladislav V. Yakovlev

Proceedings Volume 10859, Visualizing and Quantifying Drug Distribution in Tissue III; 108590H (2019) https://doi.org/10.1117/12.2510974
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

To successfully develop and manufacture a generic drug product, the latter is expected to be bioequivalent to its referencelisted drug, i.e. to show no significant difference in the rate and extent of absorption of the active pharmaceutical ingredient. Optical spectroscopy methods based on vibrational spectroscopy imaging have recently attracted significant attention as potentially viable approaches to quantify drugs’ pharmacokinetics in vivo. However, substantial hurdles still exist due to signal interference from surrounding tissues, significant attenuation of signal in the depth of tissue to optical absorption and scattering, and a lack of quantifiable ways of assessing the signal generated from a drug compound in the depth of a tissue. In this report, we evaluated the major challenges of quantification and sensitive and reproducible analysis of drug distribution in tissues using Raman spectroscopy. Specific attention is given to the noise assessment, which affects both the sensitivity and reproducibility of data.

Citation Download Citation

Sean P. O'Connor, Joshua Lalonde, Dawson T. Nodurft, and Vladislav V. Yakovlev "(Re)defining sensitivity of chemical imaging", Proc. SPIE 10859, Visualizing and Quantifying Drug Distribution in Tissue III, 108590H (4 March 2019); https://doi.org/10.1117/12.2510974

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