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Cornea is one of the collagen-rich connective tissues and plays an important role in vision. While X-ray scattering techniques are able to determine bulk structure of cornea, second harmonic generation microscopy can reveal depthdependent details of the corneal stroma comprised of a layered network of fibrillar collagen. In this work, we used Fast Fourier Transform second harmonic generation microscopy as a tool to determine the directionality of corneal stroma as a function of depth. Our results also display the position dependent difference of corneal stroma architecture.
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Sheng-Lin Lee, Yang-Fang Chen, Chen-Yuan Dong, "Fast Fourier transform second harmonic generation microscopy for probing the 3D structure of corneal lamella," Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088212 (22 February 2019); https://doi.org/10.1117/12.2507175