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Spectral imaging is heavily deployed in plant phenotyping due to its ability to infer aspects of the metabolome; however, observations of individual leaves are confounded by bidirectional reflectance effects. In this paper, we rigorously characterize these effects through the setup and implementation of a polarized Bidirectional Reflectance Distribution Function (pBRDF) Mueller matrix spectropolarimeter. We detail calibration procedures needed to correct for geometric and polarimetric aberrations, and demonstrate the pBRDF measurements for a maize (Zea mays) leaf. Furthermore, we present fixed transmission and reflection-mode measurements to monitor diurnal spectral Mueller-matrix dependencies caused by photosynthesis.
Clifton G. Scarboro,Donna J. Liebelt,Colleen J. Doherty, andMichael W. Kudenov
"Mueller matrix pBRDF Spectropolarimeter applied to measurements of Zea Mays leaves (Conference Presentation)", Proc. SPIE 11412, Polarization: Measurement, Analysis, and Remote Sensing XIV, 1141207 (27 April 2020); https://doi.org/10.1117/12.2561617
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Clifton G. Scarboro, Donna J. Liebelt, Colleen J. Doherty, Michael W. Kudenov, "Mueller matrix pBRDF Spectropolarimeter applied to measurements of Zea Mays leaves (Conference Presentation)," Proc. SPIE 11412, Polarization: Measurement, Analysis, and Remote Sensing XIV, 1141207 (27 April 2020); https://doi.org/10.1117/12.2561617