Effects of timolol in skin tissue repair: a longitudinal and multimodal in vivo imaging study with two-photon excitation fluorescence and second harmonic generation microscopy

Hongming Fan; Gabriella Santa Lucia; Alexandra Ritter; Alan Snyder; John Plante; Adam Sloger; Chelsea Rose; Steven Gannon; Yang Li; Heidy Revelo; Nicholas Strat; Carter Colwell; Amber Roberts; Manuel Valdebran M.D.; Tong Ye

doi:10.1117/12.2578544

5 March 2021 Effects of timolol in skin tissue repair: a longitudinal and multimodal in vivo imaging study with two-photon excitation fluorescence and second harmonic generation microscopy

Hongming Fan, Gabriella Santa Lucia, Alexandra Ritter, Alan Snyder, John Plante, Adam Sloger, Chelsea Rose, Steven Gannon, Yang Li, Heidy Revelo, Nicholas Strat, Carter Colwell, Amber Roberts, Manuel Valdebran M.D., Tong Ye

Author Affiliations +

Proceedings Volume 11618, Photonics in Dermatology and Plastic Surgery 2021; 116180I (2021) https://doi.org/10.1117/12.2578544
Event: SPIE BiOS, 2021, Online Only

Abstract

The non-selective beta-blocker timolol has shown promising evidence for healing chronic, recalcitrant wounds, improving scar cosmesis, and expediting the completion of secondary intention. The purpose of our pilot study is to use clinical imaging, two-photon excitement fluorescence (TPF) and second harmonic generation (SHG) microscopy to evaluate the temporal and molecular effects of timolol vs. normal saline in Sprague-Dawley rats traumatized by 5-millimeter dermal punch biopsy. Initial findings suggest timolol delays wound contraction, but advanced imaging techniques may reveal novel collagenous or vascular mechanisms by which timolol is affecting acute wound healing.

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Hongming Fan, Gabriella Santa Lucia, Alexandra Ritter, Alan Snyder, John Plante, Adam Sloger, Chelsea Rose, Steven Gannon, Yang Li, Heidy Revelo, Nicholas Strat, Carter Colwell, Amber Roberts, Manuel Valdebran M.D., and Tong Ye "Effects of timolol in skin tissue repair: a longitudinal and multimodal in vivo imaging study with two-photon excitation fluorescence and second harmonic generation microscopy", Proc. SPIE 11618, Photonics in Dermatology and Plastic Surgery 2021, 116180I (5 March 2021); https://doi.org/10.1117/12.2578544

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