In vivo visualization of retinal hypoxia using organic phosphorescent nanoparticles in laser-induced retinal vein occlusion in living rabbits

Van Phuc Nguyen; Jinsang Kim; Do Hyun Kang; Yanxiu Li; Yannis Paulus; Yingying Zeng

doi:10.1117/12.2577681

5 March 2021 In vivo visualization of retinal hypoxia using organic phosphorescent nanoparticles in laser-induced retinal vein occlusion in living rabbits

Van Phuc Nguyen, Jinsang Kim, Do Hyun Kang, Yanxiu Li, Yannis Paulus, Yingying Zeng

Author Affiliations +

Proceedings Volume 11623, Ophthalmic Technologies XXXI; 116231Y (2021) https://doi.org/10.1117/12.2577681
Event: SPIE BiOS, 2021, Online Only

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Abstract

Retinal vein occlusion (RVO) is a common cause of vision impairment and blindness. In RVO, thrombosis in a retinal vein and reduction in blood flow leads to the development of retinal hypoxia. Retinal hypoxia stimulates vascular endothelial cell growth factor (VEGF) production, resulting in the formation of neovascularization and vision loss. Currently, non-invasive imaging modalities are not available to track and quantify the tissue-level hypoxia in living animals. In this study, a novel organic room-temperature phosphorescence nanoparticle (PNPs) was developed and characterized for identification of retinal hypoxia in real-time in living rabbits. The ability of PNPs for hypoxia imaging was examined in rabbits with laser-induced RVO models (n=3). The location of retinal hypoxia was imaged before and after the intravitreal administration of PNPs at a concentration of 2.5 mg/mL and an injection dose of 50 µL. Phosphorescence imaging was acquired at different time points over 7 days alo

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Van Phuc Nguyen, Jinsang Kim, Do Hyun Kang, Yanxiu Li, Yannis Paulus, and Yingying Zeng "In vivo visualization of retinal hypoxia using organic phosphorescent nanoparticles in laser-induced retinal vein occlusion in living rabbits", Proc. SPIE 11623, Ophthalmic Technologies XXXI, 116231Y (5 March 2021); https://doi.org/10.1117/12.2577681

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