Determination of the distribution of drug concentration and tissue optical properties for ALA-mediated photodynamic therapy

Yi Hong Ong; Andrew C. Li; Theresa M. Busch; Timothy C. Zhu

doi:10.1117/12.2581824

8 March 2021 Determination of the distribution of drug concentration and tissue optical properties for ALA-mediated photodynamic therapy

Yi Hong Ong, Andrew C. Li, Theresa M. Busch, Timothy C. Zhu

Author Affiliations +

Proceedings Volume 11628, Mechanisms and Techniques in Photodynamic Therapy and Photobiomodulation; 1162806 (2021) https://doi.org/10.1117/12.2581824
Event: SPIE BiOS, 2021, Online Only

Abstract

PDT efficacy depends on the availability and dynamic interactions of photosensitizer, light, and oxygen. Tissue optical properties influence the delivered light dose and impact PDT outcome. In-vivo measurements of tissue optical properties and photosensitizer concentration enable determination of explicit and implicit dose factors affecting PDT and helps to understand the underlying biophysical mechanism of PDT. In this study, we measure tissue optical properties (absorption μa (λ) and scattering μs’ (λ) coefficients) and PpIX concentration in tissue simulating liquid phantoms with a geometry that resembles anal canal. In-vivo light fluence rate and photosensitizer fluorescence of 405nm excitation light source were acquired using a dual-motor continuous wave transmittance spectroscopy system. We characterized the tissue optical properties correction factor of fluorescence signal using a series of tissue simulating phantoms with known PpIX concentrations and with absorption coefficient between 0.1 – 0.9 cm^-1 and reduced scattering coefficient between 5 – 40 cm^-1. The results demonstrated that our spectroscopy system could determine the distribution of tissue optical properties and PPIX concentration during anal PDT.

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Yi Hong Ong, Andrew C. Li, Theresa M. Busch, and Timothy C. Zhu "Determination of the distribution of drug concentration and tissue optical properties for ALA-mediated photodynamic therapy", Proc. SPIE 11628, Mechanisms and Techniques in Photodynamic Therapy and Photobiomodulation, 1162806 (8 March 2021); https://doi.org/10.1117/12.2581824

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