Using hyperspectral near infrared spectroscopy and diffuse correlation spectroscopy to monitor the microvascular effects of phenylephrine in vivo

Laura Mawdsley; Naomi Abayomi; Ajay Rajaram; Lawrence C. M. Yip; Stephanie Milkovich; Keith St. Lawrence; Jeffrey J. L. Carson; Christopher G. Ellis; Mamadou Diop

doi:10.1117/12.2577237

5 March 2021 Using hyperspectral near infrared spectroscopy and diffuse correlation spectroscopy to monitor the microvascular effects of phenylephrine in vivo

Laura Mawdsley, Naomi Abayomi, Ajay Rajaram, Lawrence C. M. Yip, Stephanie Milkovich, Keith St. Lawrence, Jeffrey J. L. Carson, Christopher G. Ellis, Mamadou Diop

Author Affiliations +

Proceedings Volume 11639, Optical Tomography and Spectroscopy of Tissue XIV; 116390Z (2021) https://doi.org/10.1117/12.2577237
Event: SPIE BiOS, 2021, Online Only

Abstract

Phenylephrine is commonly used in cardiac surgery is common to increase mean arterial pressure without affecting cardiac output; however, its effects on the microcirculation of the brain and in skeletal muscle are unclear. The objective of this study is to use hyperspectral near infrared spectroscopy (h-NIRS) and diffuse correlation spectroscopy (DCS) to monitor the microcirculation during a phenylephrine bolus would be able to discern the timing and effects of a phenylephrine bolus on brain and skeletal muscle microvasculature. The h-NIRS subsystem uses two spectrometers and a halogen light source, and the DCS subsystem uses a long coherence diode laser (785 nm) and a single photon counting module. Probes were positioned on the left hind limb and the top of the skull, with a source-detector distance of 10mm. Data were collected from Sprague Dawley rats (n = 1, 158 g). Nine microvascular challenges were monitored by recording a two-minute baseline, then injecting 0.1mL of phenylephrine (0.1 ug/mL) intravenously, followed by collecting data for 4 additional minutes. Oxygen saturation increased by 9% in the brain and 2% in the muscle. Blood flow increased by 60% in the brain, and 17% in the muscle. This study is the first report on the use of h-NIRS and DCS to investigate the effects of phenylephrine in the microvasculature. Dissimilitude in flow response may be due to differences in regulation mechanisms. Future work will include acquiring data from both subsystems simultaneously to provide further insight into the relationship between oxygen saturation and blood flow.

Conference Presentation

Citation Download Citation

Laura Mawdsley, Naomi Abayomi, Ajay Rajaram, Lawrence C. M. Yip, Stephanie Milkovich, Keith St. Lawrence, Jeffrey J. L. Carson, Christopher G. Ellis, and Mamadou Diop "Using hyperspectral near infrared spectroscopy and diffuse correlation spectroscopy to monitor the microvascular effects of phenylephrine in vivo", Proc. SPIE 11639, Optical Tomography and Spectroscopy of Tissue XIV, 116390Z (5 March 2021); https://doi.org/10.1117/12.2577237

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available