OCE-based quasistatic elasto-spectroscopy of living and freshly excised tumor tissue for histology-like morphological segmentation and express assessment of tumor subtypes

Vladimir Y. Zaitsev; Aleksander L Matveyev; Lev Matveev; Alexander Sovetsky; Anton Plekhanov; Ekaterina Gubarkova; Marina Sirotkina; Elena V. Zagaynova M.D.; Natalia Gladkova

doi:10.1117/12.2587183

5 March 2021 OCE-based quasistatic elasto-spectroscopy of living and freshly excised tumor tissue for histology-like morphological segmentation and express assessment of tumor subtypes

Vladimir Y. Zaitsev, Aleksander L Matveyev, Lev Matveev, Alexander Sovetsky, Anton Plekhanov, Ekaterina Gubarkova, Marina Sirotkina, Elena V. Zagaynova M.D., Natalia Gladkova

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Proceedings Volume 11645, Optical Elastography and Tissue Biomechanics VIII; 116450J (2021) https://doi.org/10.1117/12.2587183
Event: SPIE BiOS, 2021, Online Only

Abstract

This report presents an overview of recent progress in the development of novel OCE-based technologies enabling non-invasive morphological segmentation of tumor tissues during their natural development and response to therapies. The approach is based on preliminary quantitative assessment of characteristic elasticity ranges corresponding to the segmented morphological constituents (“quasistatic elasto-spectroscopy”). It has been found that such constituents are characterized by fairly well separated ranges of the Young’s modulus, which makes it possible to automatically segment up to 4-6 such zones in OCE-based elasticity maps. High consistency of such OCE-based procedure with conventional morphological segmentation of histological slides in terms of percentages of the segmented-zone areas has been demonstrated. Unlike invasive, time-consuming and laborious histological studies the developed OCE-based segmentation is non-invasive and can be performed even in vivo. High accuracy and sensitivity of the OCE-based-segmentation was demonstrated in vivo for two different types of model tumors (4T1 and Ct26) treated by PDT and chemotherapies. The OCE-based analysis can readily be combined with such OCT-based extensions as texture analysis and OCT-based angiography. The latter opens the possibility to combine morphological assessment with functional characterization of tumor response to therapies. OCE-based characterization of mechanical heterogeneity and elasticity spectra of tumors has also proven to be efficient for express assessment of patients’ breast-cancer subtypes. Overall, the developed OCE-based assessment in many applications suggests a rapid and in vivo feasible alternative to conventional histology and can be efficiently used in a wide range of biomedical problems.

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Vladimir Y. Zaitsev, Aleksander L Matveyev, Lev Matveev, Alexander Sovetsky, Anton Plekhanov, Ekaterina Gubarkova, Marina Sirotkina, Elena V. Zagaynova M.D., and Natalia Gladkova "OCE-based quasistatic elasto-spectroscopy of living and freshly excised tumor tissue for histology-like morphological segmentation and express assessment of tumor subtypes", Proc. SPIE 11645, Optical Elastography and Tissue Biomechanics VIII, 116450J (5 March 2021); https://doi.org/10.1117/12.2587183

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