Fitting approach for eliminating scattered light

Sabrina Civale; Chi Zhang; Yao Wang; Samuel Chung

doi:10.1117/12.2585518

5 March 2021 Fitting approach for eliminating scattered light

Sabrina Civale, Chi Zhang, Yao Wang, Samuel Chung

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490E (2021) https://doi.org/10.1117/12.2585518
Event: SPIE BiOS, 2021, Online Only

Abstract

Unwanted background fluorescence in microscopy can occur when light from fluorescent structures scatters against nearby tissues. Bright cell bodies produce haze that mask nearby dim structures, including neuronal fibers. We have developed a method to eliminate this haze by fitting it to a simple function. This method enables clearer post-imaging visualization of axons in our in vivo imaging of neurons. We are investigating ways to increase the computation speed of our background fitting technique to allow for real-time image improvement. The implementation of our method in real-time imaging will broaden the potential applications of this fitting method.

Conference Presentation

Citation Download Citation

Sabrina Civale, Chi Zhang, Yao Wang, and Samuel Chung "Fitting approach for eliminating scattered light", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490E (5 March 2021); https://doi.org/10.1117/12.2585518

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