Three-dimensional quantification of cerebral vasculature in mouse brains with confocal microscopy

Danny Xie; Christian Crouzet; Katiana Khouri; Krystal LoPresti; Yuke Wang; Christopher Robinson; David Cribbs; Mark Fisher; Bernard Choi

doi:10.1117/12.2578057

5 March 2021 Three-dimensional quantification of cerebral vasculature in mouse brains with confocal microscopy

Danny Xie, Christian Crouzet, Katiana Khouri, Krystal LoPresti, Yuke Wang, Christopher Robinson, David Cribbs, Mark Fisher, Bernard Choi

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Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490Z (2021) https://doi.org/10.1117/12.2578057
Event: SPIE BiOS, 2021, Online Only

Abstract

Cerebral microbleeds (CMB) are deposits of blood that accumulate within the brain. An increase in CMBs is associated with an increased risk of cognitive impairment and stroke. The types of vessels associated with CMB formation remains unclear. We recently demonstrated the combined use of exogenous labels, vessel painting, and optical clearing to achieve three-dimensional views of blood vessels and CMBs. Here, we aimed to characterize brain vasculature by quantifying key vasculature-related metrics. An automated algorithm was developed to segment blood vessels within fluorescence images. An open-source neuron tracing software, neuTube, was then used to quantify blood vessel diameters.

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Danny Xie, Christian Crouzet, Katiana Khouri, Krystal LoPresti, Yuke Wang, Christopher Robinson, David Cribbs, Mark Fisher, and Bernard Choi "Three-dimensional quantification of cerebral vasculature in mouse brains with confocal microscopy", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490Z (5 March 2021); https://doi.org/10.1117/12.2578057

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KEYWORDS

Image segmentation

Brain

Confocal microscopy

Blood vessels

Algorithm development

Blood

Kidney

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