Bacterial aggregation in optical traps

Phillip Digal; Sanaz Mohammadi; Sattar Taheri-Araghi; Anna S. Bezryadina

doi:10.1117/12.2594475

1 August 2021 Bacterial aggregation in optical traps

Phillip Digal, Sanaz Mohammadi, Sattar Taheri-Araghi, Anna S. Bezryadina

Proceedings Volume 11798, Optical Trapping and Optical Micromanipulation XVIII; 117982Y (2021) https://doi.org/10.1117/12.2594475
Event: SPIE Nanoscience + Engineering, 2021, San Diego, California, United States

Conference Poster

Abstract

In this work, we study how to grow biofilms and control their growth with optical trapping techniques. In low nutrition media Bacillus subtilis (NCIB 3610) secretes extracellular polymeric substance (EPS), glue-like substance, and ultimately form a biofilm. To observe cells aggregation into clusters and the dynamics of controlled biofilm formation, we utilize an optical tweezers system built around a commercial inverted microscope. A highly focused near-infrared laser beam generated by our optical tweezers attracts cells toward the center of the laser beam, where they are trapped into clusters.

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Phillip Digal, Sanaz Mohammadi, Sattar Taheri-Araghi, and Anna S. Bezryadina "Bacterial aggregation in optical traps", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117982Y (1 August 2021); https://doi.org/10.1117/12.2594475

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