Quantitatively characterize tissue optical clearing with different agents by Mueller matrix parameters

Jiawei Song; Nan Zeng; Hui Ma

doi:10.1117/12.2601493

9 October 2021 Quantitatively characterize tissue optical clearing with different agents by Mueller matrix parameters

Jiawei Song, Nan Zeng, Hui Ma

Proceedings Volume 11900, Optics in Health Care and Biomedical Optics XI; 119000G (2021) https://doi.org/10.1117/12.2601493
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Abstract

Polarimetry is sensitive to the microstructures and has its advantages in characterizing the tissue microstructures. Our previous work on dynamic polarization changes with tissue clearing has investigated the tissue variations by using glycerol,saturated sucrose and formamide.In this study, we use a fast Mueller matrix microscope to quantitatively compare the different mechanisms of two clearing agents: glycerol and formamide. We obtain the temporal Mueller matrix images with an interval of 15 seconds and extract the temporal features of polarization parameters. Except the depolarization and linear retardance, we also analyze Mueller matrix transformation (MMT) parameters and rotation invariants derived from Mueller matrices. The former is used to characterize the microscale anisotropy of scattering media, and the latter is suitable for assessing the breaking of symmetries of scattering matrix. These Mueller matrix parameters convey the micro characteristic of biological tissues, and then help to explore and explain the microstructural changes of tissue properties by optical clearing.

Citation Download Citation

Jiawei Song, Nan Zeng, and Hui Ma "Quantitatively characterize tissue optical clearing with different agents by Mueller matrix parameters", Proc. SPIE 11900, Optics in Health Care and Biomedical Optics XI, 119000G (9 October 2021); https://doi.org/10.1117/12.2601493

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KEYWORDS

Polarization

Polarimetry

Optical clearing

Tissue optics

Microscopes

Anisotropy

Scattering

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