Single-shot dynamic line-field optical coherence elastography at 11.5 MHz

Manmohan Singh; Alexander W. Schill; Achuth Nair; Salavat R. Aglyamov; Irina V. Larin; Kirill V. Larin

doi:10.1117/12.2609979

7 March 2022 Single-shot dynamic line-field optical coherence elastography at 11.5 MHz

Manmohan Singh, Alexander W. Schill, Achuth Nair, Salavat R. Aglyamov, Irina V. Larin, Kirill V. Larin

Proceedings Volume 11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI; 119480B (2022) https://doi.org/10.1117/12.2609979
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present an ultra-fast single-shot line-field optical coherence elastography (LFOCE) technique based on a parallel line-field spectral domain optical coherence tomography system. The system was based on a Michelson-type interferometer, a supercontinuum broadband light source, and an ultra-fast 2D spectrometer. The peak sensitivity was ~102 dB, and the sensitivity roll-off was ~37 dB over 0.8 mm. The 1/e length along the line beam as measured by the signal to noise ratio (SNR) from an image of a mirror was 3.1 mm. The 1/e width across the line beam was measured by the knife-edge technique and was ~9.7 μm. The displacement stability as measured by standard deviation over 20 ms of a glass coverslip in common-path mode was 0.52 nm at an OCT SNR of 41 dB. The camera operated at a framerate of 25 kHz with 460 lateral pixels, resulting in an A-scan rate of 11.5 MHz. Validation was performed in gelatin phantoms of various concentrations, and the results corroborated well with mechanical testing. After validation in the phantoms, OCE measurements were performed in rabbit corneas in situ in the whole eye-globe configuration. The eyes were cannulated for artificial intraocular pressure (IOP) control. The elastic wave speeds in the cornea at 10, 15, and 20 mmHg were 3.03±0.05, 4.66±0.03, and 8.85±0.08 m/s, respectively. OCE measurements were also performed in an in vivo anesthetized rabbit, and the wave propagation was successfully captured. These results show the ability of the ultra-fast OCE system to measure changes in stiffness as well performing live measurements.

Citation Download Citation

Manmohan Singh, Alexander W. Schill, Achuth Nair, Salavat R. Aglyamov, Irina V. Larin, and Kirill V. Larin "Single-shot dynamic line-field optical coherence elastography at 11.5 MHz", Proc. SPIE 11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, 119480B (7 March 2022); https://doi.org/10.1117/12.2609979

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