A quartz crystal microbalance biosensor for real-time detection of Salmonella Typhimurium based on antibody immobilization

Hyun Jung Min; Hansel Mina Cordoba; Amanda J. Deering; J. Paul Robinson; Euiwon Bae

doi:10.1117/12.2623085

1 June 2022 A quartz crystal microbalance biosensor for real-time detection of Salmonella Typhimurium based on antibody immobilization

Hyun Jung Min, Hansel Mina Cordoba, Amanda J. Deering, J. Paul Robinson, Euiwon Bae

Author Affiliations +

Proceedings Volume 12120, Sensing for Agriculture and Food Quality and Safety XIV; 1212008 (2022) https://doi.org/10.1117/12.2623085
Event: SPIE Defense + Commercial Sensing, 2022, Orlando, Florida, United States

Abstract

Infection with foodborne pathogens such as Salmonella spp. is of high risk for people with a weakened immune system. Microbiological culture method has been used in general for detection of pathogens from the food matrix; however, it is time consuming and requires experience and good level of laboratory skills. In the food safety field, various techniques which allows the rapid and simple detection have been developed at the level of a user-friendly tool for detecting the foodborne pathogens. Quartz crystal microbalance (QCM) are mass-based biosensor which measures the microgram level mass changes, enabling a user to observe the presence of the pathogen simply and rapidly. When the pathogens are bound on vibrating quartz surface, the resonant frequency of a quartz crystal will be changed due to the mass of the pathogens. In this study, the QCM detected killed Salmonella Typhimurium in the range of 〖10〗^5-〖10〗^9 CFU/mL, correlating to the averaged frequency shifts. The actual concentrations of Salmonella from the culture method were compared to the difference in the resonant frequency. The QCM sensor were treated with 11-Mercaptoundecanoic acid (11-MUDA), and EDC-NHS following by antibodies and bovine serum albumin (BSA) to utilize the antibody-antigen reaction. With a usage of peristaltic pump, the solutions could be introduced to the surface while frequencies could be monitored for each step in real-time. To acquire the evidence of Salmonella, the surfaces of the quartz crystal with the fluoresce labeled antibody were captured by the fluorescence microscope. The QCM biosensor showed the possibility of detection of Salmonella in less time, compared with the conventional method.

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Citation Download Citation

Hyun Jung Min, Hansel Mina Cordoba, Amanda J. Deering, J. Paul Robinson, and Euiwon Bae "A quartz crystal microbalance biosensor for real-time detection of Salmonella Typhimurium based on antibody immobilization", Proc. SPIE 12120, Sensing for Agriculture and Food Quality and Safety XIV, 1212008 (1 June 2022); https://doi.org/10.1117/12.2623085

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