The novel photosensitizer galactose: lutetium (Lu)-phthalocyanine for photodynamic therapy in tumor-bearing mice

Alla Bucharskaya; Galina Maslyakova; Nikita Navolokin; Georgy Terentyuk; Vanya Mantareva; Vadim Genin; Elina Genina; Valery Tuchin

doi:10.1117/12.2627759

29 April 2022 The novel photosensitizer galactose: lutetium (Lu)-phthalocyanine for photodynamic therapy in tumor-bearing mice

Alla Bucharskaya, Galina Maslyakova, Nikita Navolokin, Georgy Terentyuk, Vanya Mantareva, Vadim Genin, Elina Genina, Valery Tuchin

Author Affiliations +

Proceedings Volume 12192, Optical Technologies for Biology and Medicine; 121920Z (2022) https://doi.org/10.1117/12.2627759
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation

Abstract

For photodynamic therapy (PDT), galactose – Lutetium (Lu)-phthalocyanine, as a novel photosensitizer, was administered by intratumoral injection in 8 outbred albino male mice with inoculated hepatoma in a dose of 2 mg/kg. Then, tumors were irradiated through the skin by 670 nm-diode laser source with irradiation dose 200 J /cm2 applied for 1000 sec. The withdrawal of the animals from the experiment and sampling of tissues for morphological study were performed before (n=4) and 72 hrs after PDT (n=4). Tissue of the tumor was taken for morphological examination and fixed in 10% formalin. Serial paraffin sections were stained with hematoxylin-eosin and with the use of immunohistochemical staining with antibodies to proliferation marker Ki-67 and apoptotic marker BAX (Abcam, UK). 3 days after PDT with galactose- Lu-phthalocyanine, pronounced necrotic changes and inflammation reaction were observed in central tumor area, necrosis fields occupied up to 80% of the area. The dystrophic changes, reduction of proliferation degree and development of apoptosis in tumor cells were observed on tumor periphery. The decreased expression of proliferation marker Ki-67 and the increased expression of apoptotic marker BAX were noted in tumor cells after PDT.

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Alla Bucharskaya, Galina Maslyakova, Nikita Navolokin, Georgy Terentyuk, Vanya Mantareva, Vadim Genin, Elina Genina, and Valery Tuchin "The novel photosensitizer galactose: lutetium (Lu)-phthalocyanine for photodynamic therapy in tumor-bearing mice", Proc. SPIE 12192, Optical Technologies for Biology and Medicine, 121920Z (29 April 2022); https://doi.org/10.1117/12.2627759

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KEYWORDS

Photodynamic therapy

Tumors

Lutetium

Tissues

Medical research

Cell death

Inflammation

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