Tracking early tuberous sclerosis complex diseased organoid development with quantitative oblique back-illumination microscopy

Caroline E. Filan; Seleipiri Charles; Paloma Casteleiro Costa; Brian Cheng; Hang Lu; Francisco E. Robles

doi:10.1117/12.2650755

15 March 2023 Tracking early tuberous sclerosis complex diseased organoid development with quantitative oblique back-illumination microscopy

Caroline E. Filan, Seleipiri Charles, Paloma Casteleiro Costa, Brian Cheng, Hang Lu, Francisco E. Robles

Author Affiliations +

Proceedings Volume 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; 123830E (2023) https://doi.org/10.1117/12.2650755
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Quantitative oblique back-illumination microscopy (qOBM) is a label-free imaging technique that enables tomographic phase imaging of thick scattering samples with epi-illumination. Here, we apply qOBM to image three-dimensional brain organoid cell cultures of tuberous sclerosis complex (TSC) disease. We identify quantitative differences that occur between the TSC organoids and a control cell line, and discuss the implications of these differences on our understanding the development of TSC organoid cultures. These differences include disruptions in the tubular processes in the organoid, a higher degree of folding and non-spherical cell growth, and differences in the proliferating cell structures between the two groups.

Conference Presentation

Citation Download Citation

Caroline E. Filan, Seleipiri Charles, Paloma Casteleiro Costa, Brian Cheng, Hang Lu, and Francisco E. Robles "Tracking early tuberous sclerosis complex diseased organoid development with quantitative oblique back-illumination microscopy", Proc. SPIE 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, 123830E (15 March 2023); https://doi.org/10.1117/12.2650755

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