3D pathology of cleared clinical specimens using an optimized nonorthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscope (Conference Presentation)

Kevin W. Bishop; Adam K. Glaser; Lindsey A. Barner; Robert B. Serafin; Jonathan T. C. Liu

doi:10.1117/12.2648611

15 March 2023 3D pathology of cleared clinical specimens using an optimized nonorthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscope (Conference Presentation)

Kevin W. Bishop, Adam K. Glaser, Lindsey A. Barner, Robert B. Serafin, Jonathan T. C. Liu

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Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC1238504 (2023) https://doi.org/10.1117/12.2648611
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Open-Top Light-Sheet (OTLS) microscopy is an emerging technique for cleared-tissue imaging that alleviates many of the physical constraints on sample size and geometry associated with conventional light-sheet microscopes. Existing OTLS designs generally use either two orthogonal objectives or a single shared objective for illumination and collection, however these architectures have various limitations for moderate NA cleared-tissue imaging. We previously developed an alternative Nonorthogonal Dual-Objective (NODO) OTLS configuration with a number of advantages for moderate-NA imaging of cleared tissues. Here we describe our latest NODO system, which is optimized specifically for high-throughput imaging of clinical samples for 3D pathology.

Conference Presentation

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Kevin W. Bishop, Adam K. Glaser, Lindsey A. Barner, Robert B. Serafin, and Jonathan T. C. Liu "3D pathology of cleared clinical specimens using an optimized nonorthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscope (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC1238504 (15 March 2023); https://doi.org/10.1117/12.2648611

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KEYWORDS

Imaging systems

Microscopes

Pathology

Microscopy

3D image processing

Image quality

Image resolution

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