Functional live-cell mid-infrared microscopy and spectroscopy by optoacoustic and optothermal detection

Tao Yuan; Francesca Gasparin; Nasire Uluc; Vasilis Ntziachristos; Miguel A. Pleitez

doi:10.1117/12.2647779

15 March 2023 Functional live-cell mid-infrared microscopy and spectroscopy by optoacoustic and optothermal detection

Tao Yuan, Francesca Gasparin, Nasire Uluc, Vasilis Ntziachristos, Miguel A. Pleitez

Proceedings Volume 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; 1239202 (2023) https://doi.org/10.1117/12.2647779
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Mid-infrared (mid-IR) spectroscopy and optoacoustic/optothermal (OA/OT) imaging are perfectly complementary technologies to each other. Vibrational molecular excitations by mid-IR absorption are utterly de-excited in the form of heat while efficient OA/OT signal generation primarily depends on heat deposition. This synergy allows overcoming the (otherwise) persistent limitations of traditional mid-IR spectroscopy and imaging in live-cell/fresh-tissue applications— i.e., sample opacity due to water absorption. Combination of mid-IR excitation and OA/OT detection has resulted in new tools for label-free live-cell, tissues, and in vivo metabolic research. Here we discuss basic principles on mid-IR detection for spectroscopy and imaging as well as the most recent developments on mid-IR OA and OT microscopy that overcome the limitations of conventional vibrational spectroscopy for biosensing and label-free metabolic microscopy.

Conference Presentation

Citation Download Citation

Tao Yuan, Francesca Gasparin, Nasire Uluc, Vasilis Ntziachristos, and Miguel A. Pleitez "Functional live-cell mid-infrared microscopy and spectroscopy by optoacoustic and optothermal detection", Proc. SPIE 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, 1239202 (15 March 2023); https://doi.org/10.1117/12.2647779

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