Bioinformatic analysis for structure and function of Glutamine synthetase (GS) from Antarctic sea ice bacterium Pseudoalteromonas

Jiahao Ma; Guotong Xu; Le Ao; Siqi Chen; Jingze Liu

doi:10.1117/12.2661100

6 December 2022 Bioinformatic analysis for structure and function of Glutamine synthetase (GS) from Antarctic sea ice bacterium Pseudoalteromonas sp. M175

Jiahao Ma, Guotong Xu, Le Ao, Siqi Chen, Jingze Liu

Author Affiliations +

Proceedings Volume 12458, International Conference on Biomedical and Intelligent Systems (IC-BIS 2022); 124580O (2022) https://doi.org/10.1117/12.2661100
Event: International Conference on Biomedical and Intelligent Systems, 2022, Chengdu, China

Abstract

Background: Glutamine synthetases are considered to be an important enzyme class for microbial adaptation to extreme environments, and studying the Antarctic sea ice bacterial glutamine synthetases can be used to explore the molecular mechanisms of psychrophilic microorganisms. Objective: This work use bioinformatics tools to predict structure and function of Glutamine synthetase (GS) from Pseudoalteromonas sp., and to provide a theoretical basis for further study. Methods: Open reading frame (ORF) of GS sequence from Pseudoalteromonas sp. was obtained by ORF finder and was translated into amino acid residue. The structure domain was analyzed by Blast. By the method of analysis tools: Protparam, ProtScale, SignalP-4.0, TMHMM, SOPMA, SWISS-MODEL, NCBI SMART-BLAST and MAGA 7.0, the structure and function of the protein were predicted and analyzed. Results: The results showed that the sequence was GS with 468 amino acid residues, theoretical molecular weight was 51986.64 Da. The protein has the closest evolutionary status with Shewanella oneidensis. Then it had no signal peptide site and transmembrane domain. Secondary structure of GS contained 35.04% α-helix, 16.67% Extended chain, 5.34% β-turn, 42.95% RandomCoil. Conclusions: This GS was a variety of biological functions of protein that may be used as a molecular samples of microbial nitrogen metabolism in extreme environments.

Citation Download Citation

Jiahao Ma, Guotong Xu, Le Ao, Siqi Chen, and Jingze Liu "Bioinformatic analysis for structure and function of Glutamine synthetase (GS) from Antarctic sea ice bacterium Pseudoalteromonas sp. M175", Proc. SPIE 12458, International Conference on Biomedical and Intelligent Systems (IC-BIS 2022), 124580O (6 December 2022); https://doi.org/10.1117/12.2661100

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Proteins

Bioinformatics

Biological research

Bacteria

Nitrogen

Chemical analysis

Analytical research

Show All Keywords

Keywords/Phrases

Search In:

Publication Years