Network-wide neuronal activity under optical trapping of synaptic vesicles in cultured neurons

Wataru Minoshima; Taketo Yasuda; Kyoko Masui; Chie Hosokawa

doi:10.1117/12.3008362

20 September 2023 Network-wide neuronal activity under optical trapping of synaptic vesicles in cultured neurons

Wataru Minoshima, Taketo Yasuda, Kyoko Masui, Chie Hosokawa

Proceedings Volume 12606, Optical Manipulation and Structured Materials Conference; 1260613 (2023) https://doi.org/10.1117/12.3008362
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

Neurons in the brain communicate by releasing and receiving neurotransmitters at synapse. Synaptic vesicles (SVs) that encapsulate neurotransmitters play an important role for neuronal communication. We demonstrate that optical trapping of synaptic vesicles in cultured rat hippocampal neurons regulates the neuronal network activity. The neuronal electrical activity was evaluated by extracellular potential measurement using microelectrodes arrays (MEAs). When a near-infrared trapping laser was focused on synaptic vesicles labeled with FM1-43 dye, fluorescence caused by two-photon absorption was observed at the focal spot. The fluorescence intensity gradually increased during the laser irradiation time at the laser power of 500 mW, indicating that optical trapping forces cause the assembly of SVs at the focal spot. In the extracellular potential measurement of neuronal electrical activity, spike number of spontaneous neuronal activity increased under optical trapping of SVs. The synchronicity of neuronal network activity by cross-correlation analysis increased after the laser irradiation under higher laser power conditions. These results suggest that neuronal electrical activity can be manipulated by optical trapping of synaptic vesicles.

Citation Download Citation

Wataru Minoshima, Taketo Yasuda, Kyoko Masui, and Chie Hosokawa "Network-wide neuronal activity under optical trapping of synaptic vesicles in cultured neurons", Proc. SPIE 12606, Optical Manipulation and Structured Materials Conference, 1260613 (20 September 2023); https://doi.org/10.1117/12.3008362

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KEYWORDS

Optical tweezers

Neurons

Fluorescence

Laser irradiation

Fluorescence intensity

Electrodes

Neurotransmitters

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