Quantitative evaluation of nanoparticles internalization in living cells by holographic microscopy

Daniele Pirone; Martina Mugnano; Pasquale Memmolo; Francesco Merola; Giuseppe Cesare Lama; Rachele Castaldo; Lisa Miccio; Vittorio Bianco; Simonetta Grilli; Pietro Ferraro

doi:10.1117/12.2674984

11 August 2023 Quantitative evaluation of nanoparticles internalization in living cells by holographic microscopy

Daniele Pirone, Martina Mugnano, Pasquale Memmolo, Francesco Merola, Giuseppe Cesare Lama, Rachele Castaldo, Lisa Miccio, Vittorio Bianco, Simonetta Grilli, Pietro Ferraro

Author Affiliations +

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 1262217 (2023) https://doi.org/10.1117/12.2674984
Event: SPIE Optical Metrology, 2023, Munich, Germany

Conference Poster

Abstract

Nanoparticles (NPs) internalization process in living cells has large perspectives for drug-delivery applications, but the efficient cellular uptake is still a remarkable challenge. Recently, it has been demonstrated that nanographene oxide (nGO) particles are massively internalized into the cell cytoplasm, opening the way of using graphene family materials for the above goal. Internalized nGO can interact with intracellular elements, thus modifying the life cycle pathways. Therefore, there is a great interest in studying the cellular uptake process and the volumetric distribution of nGO inside the cells. He we report on the use of holographic microscopy for quantitatively evaluating the nGO cellular uptake in both 2D and 3D. In particular, quantitative phase images of adherent cells with internalized nGO are used to measure the cells biovolume variation in time, while tomographic reconstructions of cells in flow cytometry condition are exploited to visualize in 3D the distribution of nGO within the cell's cytoplasm. The study is conducted on NIH-3T3 cells to analyze the effects of nGO in vitro and monitoring the cell culture was for several hours to allow a time-lapse of nGO uptake.

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Daniele Pirone, Martina Mugnano, Pasquale Memmolo, Francesco Merola, Giuseppe Cesare Lama, Rachele Castaldo, Lisa Miccio, Vittorio Bianco, Simonetta Grilli, and Pietro Ferraro "Quantitative evaluation of nanoparticles internalization in living cells by holographic microscopy", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 1262217 (11 August 2023); https://doi.org/10.1117/12.2674984

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