High throughput imaging of entire macro-scale organs at sub-micron resolution using double-ring modulated light-sheet microscopy

Yao Zhou; Shimeng Gao; Yifan Ma; Jianchao Liu; Peng Fei

doi:10.1117/12.2683116

18 July 2023 High throughput imaging of entire macro-scale organs at sub-micron resolution using double-ring modulated light-sheet microscopy

Yao Zhou, Shimeng Gao, Yifan Ma, Jianchao Liu, Peng Fei

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Proceedings Volume 12745, Sixteenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2023); 127450S (2023) https://doi.org/10.1117/12.2683116
Event: Sixteenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2023), 2023, Haikou, China

Abstract

Rapid 3D imaging of meso- or macro-scale organs and organisms at sub-micron resolution is challenging in life sciences. We present a static light-sheet microscopy that can achieve imaging sequences at a high speed of up to 100 fps. Our method utilizes a double-ring light modulation module in the illumination optics path to produce a low sidelobe Bessel light-sheet, providing hardware benefits for high-throughput imaging. In our study of the tumor microenvironment in human lymphoma tissue, we achieved triple-color achromatic imaging and used a custom algorithm to analyze cell co-localization, cell distance, and other relevant statistical parameters. Moreover, by combining our light-sheet system with a raster, we achieved uniform high resolution across the entire sample. Our method enables various cellular analyses at the systems level and has the potential to assist scientists in solving scientific problems.

Citation Download Citation

Yao Zhou, Shimeng Gao, Yifan Ma, Jianchao Liu, and Peng Fei "High throughput imaging of entire macro-scale organs at sub-micron resolution using double-ring modulated light-sheet microscopy", Proc. SPIE 12745, Sixteenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2023), 127450S (18 July 2023); https://doi.org/10.1117/12.2683116

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KEYWORDS

Biological imaging

Microscopy

Lymphoma

Image resolution

Brain

Head

Modulation

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