Imaging the brain in vivo with reflectance phase-contrast confocal microscopy at 1650nm

P. Delafontaine Martel; Cong Zhang; Andreas A. Linninger; Frédéric Lesage

doi:10.1117/12.3001171

12 March 2024 Imaging the brain in vivo with reflectance phase-contrast confocal microscopy at 1650nm

P. Delafontaine Martel, Cong Zhang, Andreas A. Linninger, Frédéric Lesage

Proceedings Volume 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; 1284805 (2024) https://doi.org/10.1117/12.3001171
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Recent advancements in sources and detectors operating in the NIR-II wavelengths have driven the emergence of NIR-II intrinsic microscopy. These significant technological strides were necessary because longer wavelengths are known to experience reduced scattering and absorption in biological tissue. Leveraging this optical advantage, the application of the NIR-II spectral domain in microscopy holds the potential to improve the depth of imaging and preserve coherence depth. In this study, we showcase the integration of phase-contrast imaging into a NIR-II reflectance confocal microscope for cortical imaging. By capturing images of cortical cell bodies at depths of up to 800 μm, we demonstrate that the implementation of phase contrast provides clear delineation of cortical cell edges, including myelinated axons, blood vessels, and cortical cell bodies. Additionally, we devised a computational method to enhance dynamic components and generate a digitized vascular network from the acquired images.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

P. Delafontaine Martel, Cong Zhang, Andreas A. Linninger, and Frédéric Lesage "Imaging the brain in vivo with reflectance phase-contrast confocal microscopy at 1650nm", Proc. SPIE 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, 1284805 (12 March 2024); https://doi.org/10.1117/12.3001171

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