Detection and imaging of multiple immunotherapy markers in the tumor microenvironment with SERS nanoprobes for automated treatment response monitoring

Chrysafis Andreou; Konstantinos Plakas; Naxhije Berisha; Matthieu Gigoux; Suchetan Pal; Taha Merghoub; Michael Detty; Moritz Kircher

doi:10.1117/12.3000956

13 March 2024 Detection and imaging of multiple immunotherapy markers in the tumor microenvironment with SERS nanoprobes for automated treatment response monitoring

Chrysafis Andreou, Konstantinos Plakas, Naxhije Berisha, Matthieu Gigoux, Suchetan Pal, Taha Merghoub, Michael Detty, Moritz Kircher

Author Affiliations +

Proceedings Volume 12861, Frontiers in Biological Detection: From Nanosensors to Systems XVI; 1286102 (2024) https://doi.org/10.1117/12.3000956
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Detecting multiple molecular markers related to immune cells in vivo can revolutionize tumor phenotyping, monitor the effects of immunotherapy, and provide personalized treatment. Raman imaging with surface enhanced Raman scattering (SERS) nanoprobes is an optical imaging technique with high sensitivity and unparalleled multiplexing capacity. Here, we present a generalized methodology for the synthesis and visualization of SERS nanoprobes to detect multiple targets in vivo after intravenous administration in mouse models of cancer. A multiplexed imaging approach allows simultaneous scanning, unmixing, quantification, and visualization of the nanoprobes. We use this method to monitor the response of tumor-bearing mice (murine breast and colon cancer) to immune checkpoint blockade therapy. We visualize 8 immunological markers simultaneously, with subsequent immunohistological verification of each target. A spatially-aware multivariate approach is used for the segmentation of the acquired spectral images, which allowed their subsequent classification into responders or non-responders using machine learning.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Chrysafis Andreou, Konstantinos Plakas, Naxhije Berisha, Matthieu Gigoux, Suchetan Pal, Taha Merghoub, Michael Detty, and Moritz Kircher "Detection and imaging of multiple immunotherapy markers in the tumor microenvironment with SERS nanoprobes for automated treatment response monitoring", Proc. SPIE 12861, Frontiers in Biological Detection: From Nanosensors to Systems XVI, 1286102 (13 March 2024); https://doi.org/10.1117/12.3000956

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