Electron microscopy study of tumor destruction by photodynamic therapy

Mladen Korbelik; Jasenka Matisic M.D.; Gorazd Krosl

doi:10.1117/12.146323

18 June 1993 Electron microscopy study of tumor destruction by photodynamic therapy

Mladen Korbelik, Jasenka Matisic M.D., Gorazd Krosl

Proceedings Volume 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II; (1993) https://doi.org/10.1117/12.146323
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Sections of SCCVII tumor (squamous cell carcinoma) grown in C3H mice treated with Photofrin based photodynamic therapy (PDT) were examined by electron microscopy. The tumors were collected at different times after light treatment, and divided according to light penetration into superficial (treatment light oriented) and deep areas. The results show evidence of the progressive destruction of tumor cells occurring in both areas, with the presence of apparently intact tumor cells in the deep region 6 hours after PDT. These healthy looking tumor cells were often found adjacent to heavily damaged tumor cells, which is indicative of selectivity in their killing within the same microregion of tumor tissue. Many histiocytes and mast cells that appeared engaged in tumoricidal activity were also detected. It is suggested that in addition to lethality coming from progressive hemorrhagic necrosis, host immune cells infiltrating the tumor are elicited by PDT and contribute to the indirect killing effect.

Citation Download Citation

Mladen Korbelik, Jasenka Matisic M.D., and Gorazd Krosl "Electron microscopy study of tumor destruction by photodynamic therapy", Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); https://doi.org/10.1117/12.146323

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