Near-infrared FT-Raman spectroscopy of living photosynthetic bacteria

Yukihiro Ozaki; H. Sato; K. Okada; Y. Koyama; K. Uehara

doi:10.1117/12.146135

17 June 1993 Near-infrared FT-Raman spectroscopy of living photosynthetic bacteria

Yukihiro Ozaki, H. Sato, K. Okada, Y. Koyama, K. Uehara

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146135
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Near-infrared (NIR) excited FT-Raman spectra were measured in vivo for whole living photosynthetic bacteria. Rhodobacter (Rb.) sphaeroides G1C and R26 and Chlorobium (C.) limicola f. thiosulfatophilum. The obtained spectra, which were preresonanced with the Q_y bands of bacteriochlorophyll-a (BChl-a) or bacteriochlorophyll-c (BChl-c), did not contain contributions from major components of the bacteria such as proteins and lipids, and therefore, provided selectively structural information about BChl-a in the light-harvesting (LH) complexes (Rb. sphaeroides) or BChl-c in the chlorosome (C. limicola f. thiosulfatophilum) in a totally nondestructive manner.

Citation Download Citation

Yukihiro Ozaki, H. Sato, K. Okada, Y. Koyama, and K. Uehara "Near-infrared FT-Raman spectroscopy of living photosynthetic bacteria", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146135

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