10 January 1996 Development of procedures for the identification of human papilloma virus DNA fragments in laser plume
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Abstract
For the investigation of laser plume for the existence of HPV DNA fragments, which possibly occur during laser treatment of virus infected tissue, human papillomas and condylomas were treated in vitro with the CO2-laser. For the sampling of the laser plume a new method for the trapping of the material was developed by use of water-soluble gelatine filters. These samples were analyzed with the polymerase chain reaction (PCR) technique, which was optimized in regard of the gelatine filters and the specific primers. Positive PCR results for HPV DNA fragments up to the size of a complete oncogene were obtained and are discussed regarding infectiousity.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Wolfgang Woellmer, Wolfgang Woellmer, Tom Meder, Tom Meder, Uta Jappe, Uta Jappe, Gerd Gross, Gerd Gross, Sabine Riethdorf, Sabine Riethdorf, Lutz Riethdorf, Lutz Riethdorf, Christina Kuhler-Obbarius, Christina Kuhler-Obbarius, Thomas Loening, Thomas Loening, "Development of procedures for the identification of human papilloma virus DNA fragments in laser plume", Proc. SPIE 2624, Laser-Tissue Interaction and Tissue Optics, (10 January 1996); doi: 10.1117/12.229555; https://doi.org/10.1117/12.229555
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