Quantitative detection of multiple fluorophore sites as a tool for diagnosis and monitoring disease progression in salivary glands

Israel Gannot; Robert F. Bonner; Gallya Gannot; Philip C. Fox; Joon Shik You; Ronald W. Waynant; Amir H. Gandjbakhche

doi:10.1117/12.280241

18 August 1997 Quantitative detection of multiple fluorophore sites as a tool for diagnosis and monitoring disease progression in salivary glands

Israel Gannot, Robert F. Bonner, Gallya Gannot, Philip C. Fox, Joon Shik You, Ronald W. Waynant, Amir H. Gandjbakhche

Author Affiliations +

Proceedings Volume 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II; (1997) https://doi.org/10.1117/12.280241
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A series of fluorescent surface images were obtained from physical models of localized fluorophores embedded at various depths and separations in tissue phantoms. Our random walk theory was applied to create an analytical model of multiple flurophores embedded in tissue-like phantom. Using this model, from acquired set of surface images, the location of the fluorophores was reconstructed and compared it to their known 3-D distributions. A good correlation was found, and the ability to resolve fluorophores as a function of depth and separation was determined. In parallel in in-vitro study, specific coloring of sections of minor salivary glands was also demonstrated. These results demonstrate the possibility of using inverse methods to reconstruct unknown locations and concentrations of optical probes specifically bound to infiltrating lymphocytes in minor salivary glands of patients with Sjogren's syndrome.

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Israel Gannot, Robert F. Bonner, Gallya Gannot, Philip C. Fox, Joon Shik You, Ronald W. Waynant, and Amir H. Gandjbakhche "Quantitative detection of multiple fluorophore sites as a tool for diagnosis and monitoring disease progression in salivary glands", Proc. SPIE 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II, (18 August 1997); https://doi.org/10.1117/12.280241

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