Improvements to quantitative microscopy through the use of digital micromirror devices

Andrew L. P. Dlugan; Calum E. MacAulay; Pierre Mark Lane

doi:10.1117/12.384200

27 April 2000 Improvements to quantitative microscopy through the use of digital micromirror devices

Andrew L. P. Dlugan, Calum E. MacAulay, Pierre Mark Lane

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384200
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

All of the different modes of microscopy deliver light in a controlled fashion to the object to be examined and collect as much of the light containing the desired information about the object as possible. The system being presented replaces the simple circular or annular diaphragms of a conventional microscope with digital micromirror devices to enable digital light microscopy. The DMDs are placed in the optical path at positions corresponding to the field and aperture diaphragms of a conventional microscope. This allows for more precise and flexible control over the spatial location, amount, and angles of the illumination light, and the light to be collected. Digital light microscopy enables the improvement of existing modes of microscopy, specifically for quantitative microscopy applications. Confocal microscopy has been performed, realizing improvements in resolution, flexibility, and cost. Three different combinations of image acquisition and post- processing algorithms have ben sued to generate confocal images, as well as a tomographic reconstruction image.

Citation Download Citation

Andrew L. P. Dlugan, Calum E. MacAulay, and Pierre Mark Lane "Improvements to quantitative microscopy through the use of digital micromirror devices", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384200

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