Simulation of basic subcellular scattering in tissues

Juergen Spaeth; Martin Radina; Manfred D. Kessler

doi:10.1117/12.431557

4 May 2001 Simulation of basic subcellular scattering in tissues

Juergen Spaeth, Martin Radina, Manfred D. Kessler

Proceedings Volume 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II; (2001) https://doi.org/10.1117/12.431557
Event: Saratov Fall Meeting 2000, 2001, Saratov, Russian Federation

Abstract

The main signals of light coming back from tissues result from multiple scattering interactions of the incoming light wave with the multipoles of subcellular particles. For a detailed knowledge of the systems, noninvasive optical measurement techniques in the microvolume (capillary, cellular and subcellular signals) are well established at the Institute of Physiology. Due to these methods, the scattering signals and their changes can be detected and analyzed quantitatively at the micrometers ³ volume. The scattering parameters of interest can be set separately and the results can be visualized by three dimensional imaging techniques. Mitochondria produce different scattering patterns by a change of their respiratory state due to different sizes. The algorism presented simulates this scattering. It allows fast predictions of effects like variation of particle size, variation of concentration and absorption at different geometries of lightguides. A comparison of the simulation with the measurements in microvolume shows correlation so that the algorism is reliable for qualitative and quantitative explanation of what happens in the tissue. A remarkable effect is that there are no big differences between measurements in 360- degree direction and 90 degrees because of massive multiple scattering effects.

Citation Download Citation

Juergen Spaeth, Martin Radina, and Manfred D. Kessler "Simulation of basic subcellular scattering in tissues", Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); https://doi.org/10.1117/12.431557

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