Imaging microscopic fluid viscosity and velocity fields using confocal scanning optical tweezers

Boaz A. Nemet; Nili Harnik; Mark Cronin-Golomb

doi:10.1117/12.468338

28 May 2002 Imaging microscopic fluid viscosity and velocity fields using confocal scanning optical tweezers

Boaz A. Nemet, Nili Harnik, Mark Cronin-Golomb

Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468338
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Confocal microscopy and optical tweezers were combined to develop a minimally invasive instrument capable of making hydrodynamic measurements more rapidly than is possible with other devices. This result leads to the possibility of making scanning images of the viscosity distribution of materials around bipolymer producing cells. An image of the viscosity distribution around a pullulan producing cell of Aureobasidium pullulans is shown as an example. We present results from experiments supporting a linearized model for the motion of a trapped bead in an oscillating harmonic potential. Fluid velocity measurements are tested by comparing to an independent video based measurement. We apply the technique to obtain a 2-D map of the flow past a microscopic wedge and compare to a theoretical solution for the stream lines assuming potential flow. Since the velocity is measured simultaneously with the trap relaxation time, it requires practically no calibration and is independent of the trap stiffness and the particle size.

Citation Download Citation

Boaz A. Nemet, Nili Harnik, and Mark Cronin-Golomb "Imaging microscopic fluid viscosity and velocity fields using confocal scanning optical tweezers", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468338

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