AcycloPrime: a novel method for SNP analysis using fluorescence polarization

Richard A. Greene; James J. DiMeo; Mary E. Malone; Suzanne Swartwout; Jianzhao Liu; Philip R. Buzby

doi:10.1117/12.472100

21 June 2002 AcycloPrime: a novel method for SNP analysis using fluorescence polarization

Richard A. Greene, James J. DiMeo, Mary E. Malone, Suzanne Swartwout, Jianzhao Liu, Philip R. Buzby

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472100
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation between individuals of a species and are therefore thought to be responsible for a large part of individual phenotypic variation. It has been estimated that a SNP may occur every 100-300 bases in the human genome. Research on human SNPs is expected to facilitate genetic mapping studies that may lead to a better understanding of the genetic basis for complex diseases and individual variation in drug metabolism. We have developed a novel assay for the identification of known SNPs using primer extension with the novel AcycloTerminators and a new thermostable polymerase, AcycloPol, in a homogeneous fluorescence polarization (FP) format. All assay steps can be performed in the same well of either a 384- or 96-well PCR-compatible microplate. FP provides several advantages, including simplicity and low reagent cost. The homogeneous assay format eliminates any need for separation or washing steps and is amenable to automation.

Citation Download Citation

Richard A. Greene, James J. DiMeo, Mary E. Malone, Suzanne Swartwout, Jianzhao Liu, and Philip R. Buzby "AcycloPrime: a novel method for SNP analysis using fluorescence polarization", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472100

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