Determination of depth of in vivo bioluminescent signals using spectral imaging techniques

Olivier Coquoz; Tamara L. Troy; Dragana Jekic-McMullen; Bradley W. Rice

doi:10.1117/12.477885

12 September 2003 Determination of depth of in vivo bioluminescent signals using spectral imaging techniques

Olivier Coquoz, Tamara L. Troy, Dragana Jekic-McMullen, Bradley W. Rice

Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications; (2003) https://doi.org/10.1117/12.477885
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

A spectral imaging technique applied to in vivo bioluminescent imaging is presented that provides an estimate of the depth of bioluminescent reporters inside living animals. The model, based on the standard diffusion approximation of light propagating in a slab sample, is described in this paper. Validation experiments performed on phantom and tissue models, as well as preliminary in vivo mouse images, demonstrate the ability of spectral imaging to provide a correct estimate of depth based upon a single view imaging system.

Citation Download Citation

Olivier Coquoz, Tamara L. Troy, Dragana Jekic-McMullen, and Bradley W. Rice "Determination of depth of in vivo bioluminescent signals using spectral imaging techniques", Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); https://doi.org/10.1117/12.477885

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available